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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
01/10/2019 |
Actualizado : |
01/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTILLO, A.; CARRO, B. L.; DALLA RIZZA, M.; REYNO, R. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; B. LÓPEZ CARRO, Flow Cytometry and Cell Sorting Core, Institute of Biological Research Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Use of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum. |
Complemento del título : |
Conference Paper. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Acta Horticulturae, 15 November 2018, N° 1224, p. 73-80. |
Serie : |
(Acta Horticulturae; 1224) |
ISBN : |
978-94-62612-20-4 |
ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2018.1224.11 |
Idioma : |
Inglés |
Notas : |
In: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva. |
Contenido : |
ABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal
guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry.
@ISHS, International Society for Horticultural Science. MenosABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine r... Presentar Todo |
Palabras claves : |
BIOMASS; COLCHICINE; ORYZALIN; POLYPLOIDY. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02755naa a2200265 a 4500 001 1060254 005 2019-10-01 008 2018 bl uuuu u00u1 u #d 020 $a978-94-62612-20-4 022 $a0567-7572 (print) / 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2018.1224.11$2DOI 100 1 $aCASTILLO, A. 245 $aUse of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum.$h[electronic resource] 260 $c2018 490 $a(Acta Horticulturae; 1224) 500 $aIn: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva. 520 $aABSTRACT. The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry. @ISHS, International Society for Horticultural Science. 653 $aBIOMASS 653 $aCOLCHICINE 653 $aORYZALIN 653 $aPOLYPLOIDY 700 1 $aCARRO, B. L. 700 1 $aDALLA RIZZA, M. 700 1 $aREYNO, R. 773 $tActa Horticulturae, 15 November 2018, N° 1224, p. 73-80.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/03/2018 |
Actualizado : |
12/09/2019 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
PEREIRA MACHÍN, M.; LARRATEA, F. |
Afiliación : |
MARCELO PEREIRA MACHÍN; FERNANDA LARRATEA LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Campo natural, información satelital, uso de la regla. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
ln: GÓMEZ MILLER, R.; PORCILE, V. (Ed.). Mejora de la sostenibilidad de la ganadería familiar en Uruguay. Montevideo (Uruguay): INIA, 2018. |
Páginas : |
p. 49-55 |
Serie : |
(INIA Serie Técnica; 240) |
ISBN : |
978-9974-38-302-0 |
ISSN : |
1688-9266 |
DOI : |
http://doi.org/10.35676/INIA/ST.240 |
Idioma : |
Español |
Contenido : |
El campo natural es el recurso forrajero más importante que tiene el Uruguay, principal fuente de alimento de los rodeos y majadas de cría, y uno de los determinantes principales de la competitividad internacional de la cadena cárnica y lanera de nuestro país. Poder conocer el potencial productivo del recurso, su dinámica y variación es muy importante cuando se toman decisiones. Con ese objetivo se utilizó la herramienta del seguimiento forrajero vía teledetección. |
Thesagro : |
CAMPO NATURAL. |
Asunto categoría : |
E50 Sociología rural y seguridad social |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8954/1/st240-2018p49-55.pdf
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Marc : |
LEADER 01148naa a2200205 a 4500 001 1058279 005 2019-09-12 008 2018 bl uuuu u00u1 u #d 020 $a978-9974-38-302-0 022 $a1688-9266 024 7 $ahttp://doi.org/10.35676/INIA/ST.240$2DOI 100 1 $aPEREIRA MACHÍN, M. 245 $aCampo natural, información satelital, uso de la regla. 260 $c2018 300 $ap. 49-55 490 $a(INIA Serie Técnica; 240) 520 $aEl campo natural es el recurso forrajero más importante que tiene el Uruguay, principal fuente de alimento de los rodeos y majadas de cría, y uno de los determinantes principales de la competitividad internacional de la cadena cárnica y lanera de nuestro país. Poder conocer el potencial productivo del recurso, su dinámica y variación es muy importante cuando se toman decisiones. Con ese objetivo se utilizó la herramienta del seguimiento forrajero vía teledetección. 650 $aCAMPO NATURAL 700 1 $aLARRATEA, F. 773 $tln: GÓMEZ MILLER, R.; PORCILE, V. (Ed.). Mejora de la sostenibilidad de la ganadería familiar en Uruguay. Montevideo (Uruguay): INIA, 2018.
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